![]() In this article, we offer guidance on how to select the right antibody according to the multiple variables. However, time spent researching the best product to buy can save time and resources down the line and substantially reduce frustration. With an estimated total of 4.5 million commercial antibodies from 350-plus suppliers available, it can be a daunting task to choose an antibody. For rare targets, it might be difficult to find an antibody at all, while for widely studied proteins, there might be thousands of antibodies to choose from. It is, therefore, crucial to choose the right antibody from the start. 1 While maybe not the leading cause, antibodies, and reagents as a whole, are a common source of error and inaccuracy fueling the reproducibility crisis. Yet, antibodies are also at the nexus of reproducibility issues plaguing laboratories. The quality of the staining may be influenced by several variables that need to be considered to produce reliable and consistent results, for instance, antibody selection.Īntibodies are indispensable for laboratories and represent an invaluable tool with multiple applications. Although IHC is a well-established laboratory method with a long-standing history, there is a wide range of factors influencing its outcome. ![]() Immunohistochemistry (IHC) is a widely used technique to analyze the anatomy of a tissue of interest and to visualize the expression, localization, and intensity of a specific antigen. ![]()
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